SRE0005

Proteinase K from Tritirachium album

Reagents designed and manufactured under current ISO 13485 certification.

• Synonym(s): Endopeptidase K
• CAS Number: 39450-01-6
• Enzyme Commission number: 3.4.21.64 (BRENDA, IUBMB)
• EC Number: 254-457-8
• MDL number: MFCD00132129
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• grade: for molecular biology
• Quality Level: 500
• form: buffered aqueous glycerol solution
• mol wt: 28.93 kDa
• concentration: ≥10 mg/mL protein; ≥800 unit/mL
• impurities: ≤500 pg/mg DNA (PicoGreen^® assay)
• application(s): diagnostic assay manufacturing
• foreign activity: DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Unit Definition

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Legal Information

PicoGreen is a registered trademark of Life Technologies

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable