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M3003

Sigma-Aldrich

Myokinase from rabbit muscle

ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)

Synonym(s):
ATP: AMP phosphotransferase, Adenylate Kinase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

biological source

rabbit muscle

Quality Level

form

ammonium sulfate suspension

specific activity

1,500-3,000 units/mg protein (biuret)

mol wt

21 kDa

foreign activity

3-phosphoglyceric phosphokinase ≤0.05%
lactic dehydrogenase and pyruvate kinase ≤0.01%

storage temp.

2-8°C

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This Item
P3397A8811F1757
biological source

rabbit muscle

biological source

rabbit muscle

biological source

rabbit muscle

biological source

-

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

specific activity

1,500-3,000 units/mg protein (biuret)

specific activity

≥100 units/mg protein

specific activity

10-20 units/mg protein

specific activity

300-500 units/mg protein (biuret)

mol wt

21 kDa

mol wt

-

mol wt

-

mol wt

-

foreign activity

3-phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase and pyruvate kinase ≤0.01%

foreign activity

lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05%

foreign activity

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%

foreign activity

malic dehydrogenase ≤0.1%

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.

Application

Myokinase has been used in a study to assess its reaction with the mercurials p-hydroxymercuribenzoate and p-mercuribenzenesulfonate. It has also been used in a study to investigate the ligand-binding properties of its peptide fragments.

Biochem/physiol Actions

Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.
Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP
Rabbit muscle myokinase can be inactivated by treatment with dithiobis-(2-nitrobenzoic) acid which alters methionine residues critical to myokinase activity.

Unit Definition

One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution containing 0.001 M EDTA, pH 6.0

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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