M1533

Sigma-Aldrich

N,N′-Methylenebisacrylamide solution

suitable for electrophoresis, 2% in H2O

Synonym(s):
BIS
Linear Formula:
(CH2=CHCONH)2CH2
CAS Number:
Molecular Weight:
154.17
Beilstein/REAXYS Number:
1706297
MDL number:
PubChem Substance ID:
NACRES:
NB.22

Quality Level

sterility

0.2 μm filtered

form

liquid

concentration

2% in H2O

application(s)

electrophoresis: suitable

storage temp.

2-8°C

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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General description

N,N′-Methylenebisacrylamide solution is a cross-linking agent used in polyacrylamide gel preparation.

Application

N,N′-Methylenebisacrylamide solution has been used as a component of hydrogel precursor solution and magnified analysis of the proteome (MAP) gel solution during hydrogel fabrication. It has also been used as a component of re-embedding solution during re-embedding of expanded hydrogels.
To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Packaging

25, 100 mL in poly bottle

Preparation Note

Prepared from electrophoresis grade bis-acrylamide in ultra-pure water.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

hazcat

Muta. 1B

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Fabian U Zwettler et al.
Nature communications, 11(1), 3388-3388 (2020-07-09)
Expansion microscopy (ExM) enables super-resolution fluorescence imaging of physically expanded biological samples with conventional microscopes. By combining ExM with single-molecule localization microscopy (SMLM) it is potentially possible to approach the resolution of electron microscopy. However, current attempts to combine both...
Jean Guglielminotti et al.
Anesthesiology, 122(5), 985-993 (2015-03-03)
Individual assessment of the amplitude of a physiologic reflex evoked by a standardized noxious test (SNT) before surgical stimulation has been suggested to predict movement upon the forthcoming surgical stimulation. This study aimed to compare the ability of pupillary dilatation...
A J Aho et al.
British journal of anaesthesia, 115(2), 258-266 (2015-07-04)
Concomitantly recorded Bispectral Index® (BIS) and Entropy™ values sometimes show discordant trends during general anaesthesia. Previously, no attempt had been made to discover which EEG characteristics cause discrepancies between BIS and Entropy. We compared BIS and Entropy values, and analysed...
Bjørn Bjorvatn et al.
Sleep & breathing = Schlaf & Atmung, 19(4), 1387-1393 (2015-04-10)
The objective of the present study was to investigate the prevalence of insomnia and excessive sleepiness in relation to the presence and severity of obstructive sleep apnea (OSA). The sample consisted of patients referred to a university hospital on suspicion...
Barbara R Braams et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(18), 7226-7238 (2015-05-08)
Prior studies have highlighted adolescence as a period of increased risk-taking, which is postulated to result from an overactive reward system in the brain. Longitudinal studies are pivotal for testing these brain-behavior relations because individual slopes are more sensitive for...

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