A8887

Sigma-Aldrich

Acrylamide

suitable for electrophoresis, ≥99%

Synonym(s):
2-Propenamide, Acrylic acid amide
Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
Beilstein/REAXYS Number:
605349
EC Number:
MDL number:
eCl@ss:
39031205
PubChem Substance ID:
NACRES:
NB.22

Quality Level

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99%

application(s)

electrophoresis: suitable

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

suitability

suitable for electrophoresis

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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General description

Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.
Acrylamide acts as a quencher of tryptophan fluorescence. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis. Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.

Application

Acrylamide has been used:
  • to agglomerate the vimentin and keratin intermediate filament network of K562cells
  • in the preparation and characterization of soft culture substrate
  • in hydrogel synthesis
  • for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis

Acrylamide has been used:
  • for the synthesis of hydrogels
  • for the preparation of polyacrylamide gel
  • to agglomerate vimentin and keratin intermediate filament network

Packaging

2.5 kg in poly drum
100, 500 g in glass bottle
1 kg in glass bottle

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

hazcat

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

Flash Point(F)

280.4 °F - closed cup

Flash Point(C)

138 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Removal of Mercury from Shark Using Sodium Borohydride and Product Characterization
Tenuta-Filho, et al.
INTERNATIONAL JOURNAL OF FOOD PROCESSING TECHNOLOGY (2015)
The Protein Protocols Handbook (2007)
Matrix stiffness modulates formation and activity of neuronal networks of controlled architectures
Lantoine J, et al.
Biomaterials, 89, 14-24 (2016)
Use of potato flour in bread and flat bread
Flour and Breads and Their Fortification in Health and Disease Prevention, 247-259 (2011)
A large deformation viscoelastic model for double-network hydrogels
Mao Y, et al.
Journal of the Mechanics and Physics of Solids, 100, 103-130 (2017)

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