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A8887

Sigma-Aldrich

Acrylamide

suitable for electrophoresis, ≥99%

Synonym(s):
2-Propenamide, Acrylic acid amide
Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
Beilstein:
605349
EC Number:
MDL number:
eCl@ss:
39031205
PubChem Substance ID:
NACRES:
NB.22

vapor density

2.45 (vs air)

Quality Level

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99%

form

crystals

technique(s)

electrophoresis: suitable

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

suitability

suitable for electrophoresis

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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General description

Acrylamide acts as a quencher of tryptophan fluorescence.[49] Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.[49] Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.[63]
Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide.[12] This highly reactive organic compound has the ability to polymerize to form polyacrylamide.[13]

Application

Acrylamide has been used:
  • for the synthesis of hydrogels
[62][64]
  • for the preparation of polyacrylamide gel
  • [65][57]
  • to agglomerate vimentin and keratin intermediate filament network
  • [58]
    Acrylamide has been used:
    • to agglomerate the vimentin and keratin intermediate filament network of K562cells
    [58]
  • in the preparation and characterization of soft culture substrate
  • [57]
  • in hydrogel synthesis
  • [16]
  • for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis
  • [17]

    Packaging

    100, 500 g in glass bottle
    1 kg in glass bottle
    2.5 kg in poly drum

    Pictograms

    Skull and crossbonesHealth hazard

    Signal Word

    Danger

    Hazard Classifications

    Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

    Target Organs

    Peripheral nervous system

    Storage Class Code

    6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

    WGK

    WGK 3

    Flash Point(F)

    280.4 °F - closed cup

    Flash Point(C)

    138 °C - closed cup

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

    Enter Lot Number to search for Certificate of Analysis (COA).

    Certificate of Origin

    Enter Lot Number to search for Certificate of Origin (COO).

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