suitable for cell culture, BioReagent

Linear Formula:
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

product line



≥99% (TLC)




cell culture | mammalian: suitable


white to off-white


106-108 °C (lit.)


H2O: 100 mg/mL (with heating)

storage temp.


SMILES string




InChI key


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N-Acetyl-L-cysteine has been used as a component of intestinal basal medium for the culture of mouse intestinal stem cells and also as a component of expansion medium.


5, 25, 100 g in poly bottle

Biochem/physiol Actions

Antioxidant and mucolytic agent. Increases cellular pools of free radical scavengers. Reported to prevent apoptosis in neuronal cells but induce apoptosis in smooth muscle cells. Inhibits HIV replication. May serve as a substrate for microsomal glutathione transferase.


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Signal Word


Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. My media turned yellow when I added N-acetyl cysteine (NAC), Product A9165.  Why?

    The pH of a 1% solution is 2.0-2.8.  You may need to adjust the pH of the media with NaOH.  Or, prior to addition to the media, you can initially dilute the NAC with PBS, and then add it to the culture media.

  2. How stable is a solution of Product A9165, N-acetyl cysteine (NAC)?

    Aqueous solutions of cysteine oxidize to cystine on contact with air at neutral or alkaline pH. We recommend making aqueous solutions fresh.  NAC is relatively stable in acid.  Solutions in acid are stable and can be stored at -20 °C.

  3. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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N-acetylcysteine (NAC) has been in clinical practice for several decades. It has been used as a mucolytic agent and for the treatment of numerous disorders including paracetamol intoxication, doxorubicin cardiotoxicity, ischemia-reperfusion cardiac injury, acute respiratory distress syndrome, bronchitis, chemotherapy-induced toxicity...
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