cell culture | mammalian: suitable
white to off-white
106-108 °C (lit.)
H2O: 100 mg/mL (with heating)
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Eye Irrit. 2
13 - Non Combustible Solids
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The pH of a 1% solution is 2.0-2.8. You may need to adjust the pH of the media with NaOH. Or, prior to addition to the media, you can initially dilute the NAC with PBS, and then add it to the culture media.
Aqueous solutions of cysteine oxidize to cystine on contact with air at neutral or alkaline pH. We recommend making aqueous solutions fresh. NAC is relatively stable in acid. Solutions in acid are stable and can be stored at -20 °C.
Cell culture testing is performed at 0.82 g/L. The exact concentration will depend upon your application.
We have not tested the half-life of NAC solutions in our labs here at Sigma. However, this subject has been reviewed in Neuropsychopharmacology, 29, 2018-2023 (2004).
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Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
Antioxidants protect biological systems from oxidative damage produced by oxygen-containing free radicals and from redoxactive transition metal ions such as iron, copper, and cadmium.