M7279

Sigma-Aldrich

N,N′-Methylenebisacrylamide

powder, for molecular biology, suitable for electrophoresis, ≥99.5%

Synonym(s):
BIS
Linear Formula:
(CH2=CHCONH)2CH2
CAS Number:
Molecular Weight:
154.17
Beilstein/REAXYS Number:
1706297
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NB.22

Quality Level

grade

for molecular biology

assay

≥99.5%

form

powder

application(s)

electrophoresis: suitable

impurities

≤0.05% Free carboxylic acid (T)
≤50 mg/kg Total sulfur (as SO4 ) (ICP)

conductivity

≤10 μmho (2% solution)

anion traces

chloride (Cl-): ≤50 mg/kg

cation traces

Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤5 ppm

absorption

≤0.2 at 290 at 1%

foreign activity

DNase and RNase, none detected

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Application

N,N′-Methylenebisacrylamide has been used:
  • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
  • as a crosslinker to prepare tough hydrogel network
  • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Packaging

25, 100, 250 g in poly bottle

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Statements

hazcat

Acute Tox. 3 Oral - Muta. 1B

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 2

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

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Damici GM, et al.
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Advanced Materials, 30(26), 1800109-1800109 (2018)
Fundamentals and Techniques of Biophysics and Molecular Biology (2018)
Ashok Kumar et al.
Nature protocols, 5(11), 1737-1747 (2010-10-30)
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