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L3916

Sigma-Aldrich

L-Lactic Dehydrogenase from bovine heart

1000 units/mL

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Synonym(s):
Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
CAS Number:
9001-60-9
Enzyme Commission number:
1.1.1.27 (BRENDA, IUBMB)
EC Number:
232-617-8
MDL number:
MFCD00131466
NACRES:
NA.54

form

solution

specific activity

1000 units/mL

storage temp.

2-8°C

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This Item
L7525L100659747
Sigma-Aldrich

Sigma-Aldrich

L3916

L-Lactic Dehydrogenase from bovine heart

Sigma-Aldrich

Sigma-Aldrich

L7525

L-Lactic Dehydrogenase from porcine heart

Sigma-Aldrich

Sigma-Aldrich

L1006

L-Lactic Dehydrogenase from bovine heart

Sigma-Aldrich

Sigma-Aldrich

59747

Lactic Dehydrogenase, recombinant from E. coli

specific activity

1000 units/mL

specific activity

≥200 units/mg protein

specific activity

≥400 units/mg protein

specific activity

≥90 U/mg

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

General description

LDH (lactic dehydrogenase) is a glycolytic enzyme. It is found in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It comprises of five isoenzyme forms. LDH possess a tetrameric structure.

Application

L-Lactic Dehydrogenase from bovine heart has been used:
  • as a reference standard to measure the LDH (lactic dehydrogenase)
  • to quench protein-bound NADH in cells
  • to measure the lactate released by the cells in suspension

For use in enzymatic determination of lactate or pyruvate.

Biochem/physiol Actions

LDH (lactic dehydrogenase) helps in the reversible conversion of lactate to pyruvate.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Suspension in 2.2 M ammonium sulfate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Circulating biomarkers in malignant melanoma
Advances in Clinical Chemistry, 69, 47-89 (2015)
NADH fluorescence lifetime is an endogenous reporter of alpha-synuclein aggregation in live cells
Plotegher N, et al.
Faseb Journal, 29(6), 2484-2494 (2015)
Cardiac, Vascular, and Skeletal Muscle Systems
Haschek and Rousseaux's Handbook of Toxicologic Pathology (2013)
Mitochondrial angiotensin receptors in dopaminergic neurons. Role in cell protection and aging-related vulnerability to neurodegeneration
Valenzuela R, et al.
Cell Death & Disease, 7(10), e2427-e2427 (2016)
Culture Expansion in Low-Glucose Conditions Preserves Chondrocyte Differentiation and Enhances Their Subsequent Capacity to Form Cartilage Tissue in Three-Dimensional Culture
HeywoodHannah K, et al.
BioResearch Open Access, 2484-2494 (2014)
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