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59747

Sigma-Aldrich

Lactic Dehydrogenase, recombinant from E. coli

≥90 U/mg

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Synonym(s):
(S)-Lactate: NAD+ oxidoreductase, L-Lactate Dehydrogenase, Lactate
CAS Number:
9001-60-9
Enzyme Commission number:
1.1.1.27 (BRENDA, IUBMB)
EC Number:
232-617-8
MDL number:
MFCD00131466
NACRES:
NA.54

recombinant

expressed in E. coli

form

powder

specific activity

≥90 U/mg

storage temp.

−20°C

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This Item
L1006SAE0049L2500
Sigma-Aldrich

Sigma-Aldrich

59747

Lactic Dehydrogenase, recombinant from E. coli

Sigma-Aldrich

Sigma-Aldrich

L1006

L-Lactic Dehydrogenase from bovine heart

L-Lactate Dehydrogenase (LDHA) from human, recombinant, expressed in E. coli, aqueous solution

Sigma-Aldrich

SAE0049

L-Lactate Dehydrogenase (LDHA)

Sigma-Aldrich

Sigma-Aldrich

L2500

L-Lactic Dehydrogenase from rabbit muscle

form

powder

form

buffered aqueous glycerol solution

form

aqueous solution

form

ammonium sulfate suspension

specific activity

≥90 U/mg

specific activity

≥400 units/mg protein

specific activity

-

specific activity

800-1,200 units/mg protein

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

Application

Lactic Dehydrogenase, recombinant from E. coli has been used:
  • in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
  • to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
  • in in vitro DltC D-alanylation assay

Biochem/physiol Actions

Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.

Unit Definition

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Glycolysis without pyruvate kinase in Clostridium thermocellum
Olson DG, et al.
Metabolic engineering, 39, 169-180 (2017)
A partial reconstitution implicates DltD in catalyzing lipoteichoic acid D-alanylation
Wood BM, et al.
The Journal of Biological Chemistry, 293(46), 17985-17996 (2018)
Cardiac, Vascular, and Skeletal Muscle Systems
Haschek and Rousseaux's Handbook of Toxicologic Pathology (2013)
Circulating biomarkers in malignant melanoma
Advances in Clinical Chemistry, 69, 47-89 (2015)
An atypical glycolysis in Clostridium thermocellum
Zhou J, et al.
Applied and Environmental Microbiology, 39, AEM-04037 (2013)
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