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L2625

Sigma-Aldrich

L-Lactic Dehydrogenase from bovine heart

Type III, ammonium sulfate suspension, ≥500 units/mg protein

Synonym(s):
Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type III

Quality Level

form

ammonium sulfate suspension

specific activity

≥500 units/mg protein

UniProt accession no.

foreign activity

pyruvate kinase ≤0.03%

storage temp.

2-8°C

Gene Information

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General description

Lactic dehydrogenase (LDH) catalyzes the conversion of lactate to pyruvate in anaerobic glycolysis. It exists as tetramer and comprises of two subunits (H and M).[77] The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality.[03]

Application

L-Lactic Dehydrogenase from bovine heart has been used as a standard in cytotoxicity assay.[05] It has also been used in glutamic pyruvic transaminase (GPT) assay.[06]

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Crystalline suspension in 2.1 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Technical Information

SDS

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