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K0254

Sigma-Aldrich

Kanamycin solution from Streptomyces kanamyceticus

50 - 60 mg/mL in 0.9% NaCl, BioReagent, liquid, 0.1 μm filtered, suitable for cell culture

Synonym(s):
Kanamycin A
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.76

biological source

Streptomyces kanamyceticus

Quality Level

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

concentration

50 - 60 mg/mL in 0.9% NaCl

technique(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

impurities

endotoxin, tested

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H36N4O11/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17/h4-18,23-29H,1-3,19-22H2/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-/m1/s1

InChI key

SBUJHOSQTJFQJX-NOAMYHISSA-N

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General description

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 2 mL/L.

Application

Kanamycin solution from Streptomyces kanamyceticus has been used:
  • to induce deafness in neonatal rats
[03]
  • as an endothelial growth medium component for culturing human umbilical vein endothelial cells (HUVECs)
  • [04]
  • as medium component for Mycobacteria Cultures and Top10 E coli cells
  • [06]

    Biochem/physiol Actions

    Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

    Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

    Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

    Caution

    Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

    Pictograms

    Health hazard

    Signal Word

    Danger

    Hazard Statements

    Precautionary Statements

    Hazard Classifications

    Repr. 1B

    Storage Class Code

    6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

    WGK

    WGK 2

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

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    Certificate of Origin

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