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I4381

Sigma-Aldrich

IgG from porcine serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym(s):

Pig IgG

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

unconjugated

grade

reagent grade

assay

≥95% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

technique(s)

ELISA: suitable

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG from porcine serum specifically recognizes IgG from pig. Pig IgG is purified from normal pig serum by fractionation and ion-exchange chromatography. Immunoglobulin G (IgG) is part of the immunoglobulin family. The primary structure of this antibody contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains.

Application

IgG from porcine serum may be used in quantitative ELISA, a concentration of 0.5 μg/ml may be used as standard. Concentrations ranging from 3.9 to 250 ng/ml were used as standards in immune response measurements. Pig IgG was also used as standard in surface plasmon resonance phase imaging studies.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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E van Heugten et al.
Journal of animal science, 72(3), 658-664 (1994-03-01)
To investigate the effect of aflatoxin (AF) and dietary methionine (MET) on immune responses of swine, a total of 288 pigs weaned at 21 d of age were allotted to 12 dietary treatments arranged in a 3 x 4 factorial
Kuo-Hoong Lee et al.
Biosensors & bioelectronics, 23(4), 466-472 (2007-07-10)
This study reports a microfluidic chip integrated with an arrayed immunoassay for surface plasmon resonance (SPR) phase imaging of specific bio-samples. The SPR phase imaging system uses a surface-sensitive optical technique to detect two-dimensional (2D) spatial phase variation caused by
Michael Bailey et al.
Clinical and diagnostic laboratory immunology, 11(2), 337-343 (2004-03-12)
Normal piglets weaned onto soy- or egg-based diets generated antibody responses to fed protein. Concurrent infection with transmissible gastroenteritis virus (TGEV) did not affect the responses to dietary antigens at weaning, nor did it affect the subsequent development of tolerance.
Kateryna Goncharova et al.
PloS one, 12(6), e0180002-e0180002 (2017-06-29)
Neurological disorders are among the main clinical problems affecting preterm children and often result in the development of communication and learning disabilities later in life. Several factors are of importance for brain development, however the role of immunoglobulins (passive immunity
Antibody structure, instability, and formulation.
Wang W
Journal of Pharmaceutical Sciences, 96(1), 1-26 (2007)

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