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F4135

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Synonym(s):
HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum
MDL number:
eCl@ss:
32160409
NACRES:
NA.71

biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation.[14] It also protects cells from oxidative damage and apoptosis.[51] FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.[84]

Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:
  • as a component of Dulbecco′s Modified Eagles Media (DME, DMEM),
[57] Eagle′s Minimum Essential Medium (EMEM),[89] Roswell Park Memorial Institute medium (RPMI-1640),[93] α-modified eagle medium (αMEM) (MEM)[25] and Leibovitz L-15 medium[92]
  • as a component of blocking medium for treating brainstem slices
  • [09]
  • to improve attachment of dermal explants
  • [39]

    Preparation Note

    Prepared by heating at 56°C for 30 minutes.
    This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

    Analysis Note

    • Endotoxin and hemoglobin tested
    • Tested for the presence of bacteria, virus, and mycoplasma
    • Triple filtered with 0.1 micron membrane under aseptic conditions

    Storage Class Code

    10 - Combustible liquids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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