flow cytometry: suitable
proximity ligation assay: suitable
λex 495 nm; λem 527 nm
suitable for fluorescence
10 - Combustible liquids
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of protein-protein interactions with flow cytometry readouts a reality.
General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.