In the Duolink®
PLA Control Kit, the cells and antibodies are provided, and the conditions have been optimized. When combined with the necessary Duolink®
PLA reagents, you will have all you need to successfully perform the PLA experiment and obtain positive results.
The Control kit contains Duolink®
PLA Control Slides (Cat. No. DUO82202) - two 8 well glass slides with fixed SK-OV3 Human ovarian cancer cells. The cells were treated with EGF, to induce EGFR and HER2 interaction. The Kit also contains one vial of Anti-HER2 antibody produced in rabbit (HPA001383), and one vial of Monoclonal Anti-EGFR antibody produced in mouse (E3138) – the two primary antibodies you need to detect the two interacting protein
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Based on proximity ligation assay (PLA), the Duolink® PLA Technology
allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells.
PLA Control Kit - PPI is designed to allows users to gain confidence with the Duolink®
PLA technology. This Kit contains glass slides with fixed, treated cells and a specific pair of primary antibodies that will detect interacting proteins and reliably generate PLA signals when used with the complementary Duolink®
PLA probes and detection reagents. Analysis is carried out using standard immunofluorescence assay equipment.Specificity
This kit will enable the detection of Protein-Protein interactions. The SK-OV-3 cells contain HER2 and EGFR, which show increased interaction upon treatment with EGF. When combined with appropriate Duolink®
PLA Reagents, the Control Kit will enable the successful detection of increased HER2 and EGFR protein interactions in the EGF treated cells.