DUO92006
donkey (polyclonal)
affinity purified immunoglobulin (secondary antibody)
primary antibodies
Duolink®
goat
immunofluorescence: suitable
proximity ligation assay: suitable
suitable for brightfield
suitable for fluorescence
wet ice
2-8°C
10 - Combustible liquids
WGK 3
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Things to consider for preparation, setup and execution of the Duolink® assay protocol
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This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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