Duolink® Proximity Ligation Assay
Inside every cell, there is a breakthrough waiting to be uncovered. The chance to change the origin of a human disease or discover the tell-tale signal of an irregular protein-protein interaction requires powerful investigative tools and a streamlined approach. Rely on Duolink® PLA to amplify protein signals by 1000x, allowing you to visually detect proteins and their interactions, all with a straightforward protocol.
Uncover the opportunities in your research with amplified performance and the benefits of Duolink® PLA technology:
- Visualize protein interactions – both stable and transient
- Detect endogenous proteins – no overexpression or genetic manipulation
- High specificity – use of two antibodies/probes eliminates false positives
- Single molecule sensitivity – rolling circle amplification makes proteins visible
- No special equipment needed – standard immunofluorescence methods
- Publication-ready results – compare image results from indirect methods (Co-IP/WB)
Duolink® PLA Products
- Duolink® PLA Starter Kits contains all the necessary reagents you need to perform a fluorescent experiment and analyze up to 30 samples.
- Duolink® Probemaker Kits enable the conjugation of a PLA oligo (PLUS or MINUS) to any antibody.
- Duolink® PLA Multicolor Kits allow for multiplex applications.
- Duolink® Fluorescent Kits enable use with four different fluorophores: green, orange, red, and far red.
- Duolink® flowPLA Detection Kits enables flow cytometry applications using four colors: green, orange, red, and far red.
- Duolink® PLA Brightfield Detection contains oligonucleotides labeled with horseradish peroxidase (HRP) allowing use with a light microscope.
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