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Duolink® Proximity Ligation Assay

Inside every cell, there is a breakthrough waiting to be uncovered. The chance to change the origin of a human disease or discover the tell-tale signal of an irregular protein-protein interaction requires powerful investigative tools and a streamlined approach. Rely on Duolink® PLA to amplify protein signals by 1000x, allowing you to visually detect proteins and their interactions, all with a straightforward protocol.

Uncover the opportunities in your research with amplified performance and the benefits of Duolink® PLA technology:

  • Visualize protein interactions – both stable and transient
  • Detect endogenous proteins – no overexpression or genetic manipulation
  • High specificity – use of two antibodies/probes eliminates false positives
  • Single molecule sensitivity – rolling circle amplification makes proteins visible
  • No special equipment needed – standard immunofluorescence methods
  • Publication-ready results – compare image results from indirect methods (Co-IP/WB)

Duolink® PLA Products

  • Duolink® PLA Starter Kits contains all the necessary reagents you need to perform a fluorescent experiment and analyze up to 30 samples.
  • Duolink® Probemaker Kits enable the conjugation of a PLA oligo (PLUS or MINUS) to any antibody.
  • Duolink® PLA Multicolor Kits allow for multiplex applications.
  • Duolink® Fluorescent Kits enable use with four different fluorophores: green, orange, red, and far red.
  • Duolink® flowPLA Detection Kits enables flow cytometry applications using four colors: green, orange, red, and far red.
  • Duolink® PLA Brightfield Detection contains oligonucleotides labeled with horseradish peroxidase (HRP) allowing use with a light microscope.

Related Product Resources

Brochure: Amplified Detection Duolink® Proximity Ligation Assay (PLA)
User Manual: Duolink® PLA Fluorescence Protocol
User Manual: Duolink® PLA Brightfield Protocol
Duolink® PLA Flow Cytometry Kits

How to Create PLA® Probes
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® Proximity Ligation Assay Instructional Video

Products

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Showing 1-20 of 43
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Showing 1-20 of 43
Product Number
Product Description
Pricing
DUO92002
Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)
DUO92004
Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H+L)
DUO92008
Duolink® In Situ Detection Reagents Red
DUO92101
Duolink® In Situ Red Starter Kit Mouse/Rabbit
DUO92007
Duolink® In Situ Detection Reagents Orange
DUO82049
Duolink® In Situ Wash Buffers, Fluorescence
DUO92102
Duolink® In Situ Orange Starter Kit Mouse/Rabbit
DUO92014
Duolink® In Situ Detection Reagents Green
DUO92001
Duolink® In Situ PLA® Probe Anti-Mouse PLUS
DUO92005
Duolink® In Situ PLA® Probe Anti-Rabbit MINUS
DUO92013
Duolink® In Situ Detection Reagents FarRed
DUO82040
Duolink® In Situ Mounting Medium with DAPI
DUO94005
Duolink® flowPLA Detection Kit - Violet, Duolink® PLA kit for Flow Cytometry with Violet Detection
DUO92006
Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)
DUO92012
Duolink® In Situ Detection Reagents Brightfield
DUO92009
Duolink® In Situ Probemaker PLUS
DUO92010
Duolink® In Situ Probemaker MINUS
DUO94004
Duolink® flowPLA Detection Kit - FarRed, Duolink® PLA kit for Flow Cytometry with FarRed Detection
DUO92202
Duolink® PLA Control Kit - PPI, Control Kit to reliably detect Protein-Protein Interactions (PPI) with Duolink® PLA
DUO92003
Duolink® In Situ PLA® Probe Anti-Goat PLUS
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