Prestige Antibodies® Powered by Atlas Antibodies
With industry-leading antibody characterization and lot-specific testing, our polyclonal and monoclonal Prestige Antibodies® are designed to detect and target over 80% of the human proteome in immunohistochemistry (IHC), immunofluorescence (IF), Western blot (WB), and other applications. Prestige Antibodies® undergo some of the most stringent validation and characterization available. Prestige Antibodies® are developed using open-access data from the Human Protein Atlas and validated using IHC, WB, ICC-IF, or RNA sequencing and compared to bioinformatic information and literature. Prestige Antibodies® are characterized via tissue microarrays in over 40 different normal human tissues, over 50 cell lines, and over 20 cancer types.
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What Makes Prestige Antibodies® Unique?
All Prestige Antibodies® undergo in-depth validation as well as characterization using the immense amount of supporting data from the Human Protein Atlas project. The low cross reactivity for Prestige Antibodies® to other proteins is the result of careful selection of antigen regions, affinity purification, and are validated and characterized in each application that the antibody has been demonstrated for use. Each antibody is characterized on a lot-to-lot basis with application-specific validation that includes IHC, IF, WB, and or protein array assays.
Enhanced Validation – Another Reason to Trust Prestige Antibodies®
In addition to the standard antibody validation methods to validate and characterize Prestige Antibodies®, we offer enhanced validation which is aligned to the pillars for antibody validation described by the International Working Group for Antibody Validation (IWGAV) for numerous antibodies. Many Prestige Antibodies® undergo one or more enhanced validation methods including genetic strategies, independent antibody verification, orthogonal validation using RNA-seq, and functional assay validation. Learn more about our Enhanced Validation.
IF Analysis
Western Blot
BioInformatics & Literature Search
IHC Analysis
Protein Array
For Research Use Only. Not for use in diagnostic procedures.
Unless otherwise stated in the Product(s) specifications, any Antibody product is sold for internal research use only and may not be used for any other purpose, which includes but is not limited to, any commercial, diagnostic, or therapeutic use. Our validation processes pertain only to research uses and do not confirm or assure that our antibodies can be used for any unauthorized uses as set forth herein.
Related Resources
- Brochure: Prestige Antibodies® Pan-Cancer Markers
Explore our Prestige Antibodies® portfolio classified by tumor type.
- Article: Human Protein Atlas
The human protein atlas has an aim of mapping all human proteins within cells, tissues and organs and providing open-access information to advance understanding of human biology and disease.
- Article: Prestige Antibodies® on The Cell Atlas
Visualize the spatial distribution of proteins in cells using the Cell Atlas part of the Human Protein Atlas. For each protein, there are multiple immunofluorescent staining images in a vast number of cell lines.
- Protocol: Immunohistochemistry Procedure
We presents a protocol for the Prestige Antibody Immunohistochemistry Procedure. The Prestige Antibodies are subjected to a standardized test procedure using specially designed tissue microarray (TMA) slides.
- Protocol: Immunofluorescence Procedure
We presents a protocol for Immunofluorescence Procedure. A large number of the Prestige Antibodies have been used in subcellular localization studies by immunofluorescence (IF) staining of three cell lines: A-431, U-2 OS, and U-251MG.
- Protocol: Prestige Antigens™ Blocking Protocol
Prestige Antigens™ and the corresponding Prestige Polyclonals or Monoclonals are recommended to use according to the standard Prestige Antigen-blocking protocol
- Article: Stem Cell Markers and Antibodies
Stem cell markers, including embryonic stem cell, pluripotency, transcription factors, induced PSCs, germ cells, ectoderm, and endoderm markers.
- Article: Neural Stem Cell Markers and Antibodies
Neural stem cell markers and antibodies for prominent neural cell types and targets, including neurons, glial cells, neuronal differentiation markers, Nestin, and SOX2.
- Article: Mesenchymal Stem Cell Markers and Antibodies
Mesenchymal stem cell markers and antibodies suitable for investigating targets in fibroblasts, chondrocytes, adipocytes, osteoblasts, and muscle cells.
- Brochure: Prestige Antibodies® in Neuroscience
The Human Protein Atlas project has created a complete map of protein expression in all major organs and tissues in the human body.
- Article: Prestige Antibodies® in Breast Cancer Research
The Human Protein Atlas; Prestige Antibodies®; Polyclonals and Monoclonals; Clinical Markers; Antibodies used in Breast Cancer Research; Antibodies Against MammaPrint and Other Gene Expression Test Proteins; Antibodies Identified in the Human Protein Atlas; Finding Cancer Biomarkers, as Exemplified by RBM3, Granulin and Anillin
- Brochure: Prestige Antibodies® in Colorectal Cancer Research
The Human Protein Atlas project has created a complete map of protein expression in all major organs and tissues in the human body.
- Protocol: Prestige Antibodies® in Western Blot Applications
Sigma-Aldrich.com presents a white paper on Prestige Antibodies® Powered by Atlas Antibodies in Western Blot Application
- Protocol: Prestige Antibodies® in Immunofluorescence Applications
One major rationale for investigating the subcellular location of a specific protein is that location is often tightly connected to function. For example, proteins locating to the nucleus are frequently implicated in gene regulation, proteins in mitochondria with energy production and Golgi-related proteins are often associated with protein modification and sorting.
- Article: Prestige Antibodies® in Organelle Markers
Subcellular localization studies are important for mapping and characterizing proteins and thus for better understanding of the cellular functions of the proteins. By confocal microscopy analysis on human cell lines, spatial and temporal protein expression patterns can be visualized on a fine cellular and subcellular level.
- Prestige Antibodies® Powered by Atlas Antibodies in Immunohistochemistry
Immunohistochemistry (IHC) is the most widely used technique in histopathological diagnosis and research for detection of proteins in tissues and cells. Today, IHC can be applied in a high-throughput fashion for studying proteins by the use of Tissue Microarrays (TMAs). In the Human Protein Atlas Project, Prestige Antibodies have been used to analyze more than 8,000 human proteins using IHC and TMAs
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