Cell Line Origin
Human colorectal adenocarcinoma
DMEM (High glucose Sigma D6546) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.05% trypsin/EDTA; 7.5% CO2; 37 °C.Suggested seeding density 2-4x10,000 cells/cm2.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.