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HCA-2

06061901, from human colon

Synonym(s):
HCA 2, HCA2

biological source

human colon

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Related Categories

Cell Line Origin

Human sigmoid colon adenocarcinoma

Cell Line Description

Established from a sigmoid colon adenocarcinoma of a 83 year-old female patient. The adenocarcinoma was well differentiated and determined to be Dukes′ stage C. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.

DNA Profile

Not specified

Culture Medium

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Split subconfluent cultures (70-80%) 1:2 to 1:10 using 0.25% trypsin/EDTA; 7.5% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

Certificate of Origin

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