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HT29-MTX-E12

from human colon, 12040401

Synonym(s):
HT-29-MTX-E12 Cells, HT29, HT29 Cells, HT29-MTX, HT29-MTX Cells, MTX-E12 Cells

biological source

human colon

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption

Cell Line Description

HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and productio

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17

Culture Medium

DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm².

Other Notes

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Certificate of Analysis

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Certificate of Origin

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