Cell Line Origin
Human colon adenocarcinoma
Cell Line Description
Although originally described as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29. HT29 was isolated from a primary colon adenocarcinoma in a 44 year old Caucasian female. The cells produce carcinoembryonic antigen and are tumorigenic in xenogeneic animals. They have been used in studies of anti-tumour agents.
Carcinogenesis and tumourigenicity studies and anti-tumour testing
STR-PCR Data: Amelogenin: X
vWA: 17,19 STR-PCR Data: Amelogenin: X
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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