All Photos(2)

COLO 201


biological source

human colon

growth mode



Triploid modal no. 71-78


Clumps of spherical cells in suspension, cuboidal cells in the monolayer


Carcinoembryonic antigen (CEA) less than produced by COLO 205


Not specified


cell culture | mammalian: suitable

relevant disease(s)


shipped in

dry ice

storage temp.


Related Categories

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

COLO 201, COLO 205 and COLO 206 (ECACC Catalogue 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 201 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 201 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 10
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain suspension cells at 3-9x105 cells/cm3. Split sub-confluent cultures (70-80%) of attached cells 1:3 to 1:6 i.e. seeding at 2-3x104 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificate of Analysis

Certificate of Origin

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