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A4679

Sigma-Aldrich

Agarose

Low EEO, for Immunoelectrophoresis

CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

biological source

algae (Gelidium)

Quality Level

form

powder

technique(s)

electrophoresis: suitable
immunodiffusion: suitable
immunoelectrophoresis: suitable

impurities

≤7% water

EEO

0.09-0.13

mp

88 °C±2 °C (1.5% gel)

transition temp

gel point 34-38 °C (1.5% gel)

gel strength

≥1200 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.20%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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General description

Agarose contains β-D-galactose and 3,6-anhydro-α-L-galactose, linked by glycosidic bonds β(1-4).

Packaging

50, 100, 500 g in poly bottle

Application

Agarose is the most popular medium for immunoelectrophoresis because of the large pore size for rapid diffusion and for low background staining by Coomassie Blue G stain. The low EEO and high gel strength is specifically selected and tested for immunoelectrophoresis and immunodiffusion.

Preparation Note

Gel Preparation:
1. Prepare a 1% agarose solution (sufficient for 10 gels 85 mm x 100 mm, 1-1.5 mm thick) by mixing 1.5 g agarose (Catalog No. A4679) in 150 mL of prepared barbital buffer and heat in a boiling water bath until completely dissolved.
2. To prepare one gel, pour 14 mL of agarose solution onto the hydrophilic side of a level, well supported 85 mm x 100 mm sheet of Electrophoresis Film for Agarose Gels (Catalog No. E0264). Pour from the center of the sheet toward its edges forming an even layer of agarose 1-1.5 mm thick.
3. Allow the gels to harden for one hour at 4 °C before using or store at 0-5 °C in an appropriate, plastic wrapped container.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Agarose and its derivatives as supports for enzyme immobilization
Zucca P, et al.
Molecules (Basel), 21(11), 1577-1577 (2016)
Maria Carmen Piñon et al.
The Journal of physiology, 587(Pt 9), 1903-1915 (2009-03-18)
In the Golli-tau-eGFP (GTE) transgenic mouse the reporter gene expression is largely confined to the layer of subplate neurons (SPn), providing an opportunity to study their intracortical and extracortical projections. In this study, we examined the thalamic afferents and layer
Víctor Carriel et al.
Journal of neural engineering, 10(2), 026022-026022 (2013-03-27)
The objective was to study the effectiveness of a commercially available collagen conduit filled with fibrin-agarose hydrogels alone or with fibrin-agarose hydrogels containing autologous adipose-derived mesenchymal stem cells (ADMSCs) in a rat sciatic nerve injury model. A 10 mm gap
Nathan A Baird et al.
PloS one, 3(10), e3376-e3376 (2008-10-15)
Single nucleotide polymorphism (SNP) discovery and genotyping are essential to genetic mapping. There remains a need for a simple, inexpensive platform that allows high-density SNP discovery and genotyping in large populations. Here we describe the sequencing of restriction-site associated DNA
Gianluca Vadalà et al.
Spine, 38(6), E319-E324 (2013-01-18)
Descriptive anatomical study on ovine and human cadaveric lumbar spinal segments. To describe the alternative transpedicular approach to deliver therapeutic agents into intervertebral disc (IVD). The present delivery approach of therapeutic agents (growth factors/cells/hydrogels) within the IVD is through injection

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