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A4418

Sigma-Aldrich

Ammonium sulfate

for molecular biology, ≥99.0%

Synonym(s):
Mascagnite
Linear Formula:
(NH4)2SO4
CAS Number:
Molecular Weight:
132.14
EC Number:
MDL number:
eCl@ss:
38030408
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology

Quality Level

Assay

≥99.0%

form

powder

impurities

≤0.005% Insolubles

pH

5.0-6 (25 °C, 132 g/L)

mp

>280 °C (dec.) (lit.)

solubility

H2O: 0.667 g/mL

density

1.77 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 ppm

cation traces

Cu: ≤5 ppm
Fe: ≤5 ppm
Zn: ≤5 ppm
heavy metals (as Pb): ≤2 ppm

absorption

≤0.025 at 280 at 1 M
≤0.03 at 260 at 1 M

foreign activity

DNase, RNase, and protease, none detected

SMILES string

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI key

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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Ammonium sulfate for molecular biology, ≥99.0%

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Ammonium sulfate

grade

for molecular biology

grade

-

grade

anhydrous

grade

ACS reagent

assay

≥99.0%

assay

≥99.0%

assay

≥99.0%

assay

≥99.0%

form

powder

form

powder or crystals

form

powder

form

powder or crystals

impurities

≤0.005% Insolubles

impurities

-

impurities

-

impurities

≤0.005% Insoluble matter

pH

5.0-6 (25 °C, 132 g/L)

pH

5-6 (25 °C, 132 g/L)

pH

5-6 (25 °C, 132 g/L)

pH

5.0-6.0 (25 °C, 5%)

mp

>280 °C (dec.) (lit.)

mp

>280 °C (dec.) (lit.)

mp

>280 °C (dec.) (lit.)

mp

>280 °C (dec.) (lit.)

General description

Ammonium sulfate is commonly used in agriculture as a nitrogen fertilizer. In aqueous solution, it dissociates to form ammonium and sulfate ions.

Application

Ammonium sulfate has been used in the preparation of ′Blue Silver′ colloidal Coomassie stain for proteins.
Ammonium sulfate may be used for the precipitation or fractionation of proteins or for purification of antibodies. It is also useful for crystallographic analysis of nucleic acids and proteins.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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