09978

Ammonium sulfate

BioUltra, ≥99.0% (T)

• Synonym(s): Mascagnite
• Linear Formula: (NH₄)₂SO₄
• CAS Number: 7783-20-2
• Molecular Weight: 132.14
• EC Number: 231-984-1
• MDL number: MFCD00003391
• eCl@ss: 38030408
• PubChem Substance ID: 329748870
• NACRES: NA.26

Properties

• product line: BioUltra
• Quality Level: 200
• Assay: ≥99.0% (T)
• form: crystals
• impurities: insoluble matter, passes filter test
• ign. residue: ≤0.01% (as SO₄)
• loss: ≤0.1% loss on drying, 110 °C
• pH: 5.0-6.0 (25 °C, 1 M in H₂O)
• mp: >280 °C (dec.) (lit.)
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• density: 1.77 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 mg/kg; nitrate (NO₃^-): ≤50 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.2 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤1 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤2 mg/kg; Fe: ≤2 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤50 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤2 mg/kg; Sr: ≤5 mg/kg; Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.037; λ: 280 nm A_max: ≤0.030
• SMILES string: N.N.OS(O)(=O)=O
• InChI: 1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)
• InChI key: BFNBIHQBYMNNAN-UHFFFAOYSA-N

Description

General description

Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.

Application

Ammonium sulfate has been used:

• to prepare mobile phase A for hydrophobic interaction chromatography (HIC)
• as reference material for the thermal decomposition of NH₄NO₃ (S1–S6) thermal decomposition to N₂O (S1-N₂O–S6-N₂O)
• to prepare the stock solution for M63 medium
• as a reagent in molecular biology and chromatography
• in the precipitation and fractionation of proteins
• in the purification of antibodies
• for the crystallographic analysis of nucleic acids and proteins
• in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography
• for Sepharose chromatography of tRNAs using reverse salt gradients
• in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis
• HPLC analysis of phospholipids

Biochem/physiol Actions

Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.

Other Notes

The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose^® chromatography of tRNAs using reverse salt gradients.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves