MilliporeSigma
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A7431

Sigma-Aldrich

Agarose

BioReagent, for molecular biology, Wide range/Standard 3:1

CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

biological source

algae (marine)

grade

for molecular biology

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

EEO

≤0.13

mp

≤90 °C

transition temp

gel point 32.5-38 °C (4% gel)

gel strength

≥1200 g/cm2 (4% gel)

anion traces

sulfate (SO42-): ≤0.15%

foreign activity

DNase, RNase, NICKase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Packaging

25, 100 g in poly bottle

Application

Specially formulated to form strong, flexible gels for separation of small (≤1 kb) PCR products, DNA and RNA. Stronger gels make gel-handling easier. Sharp resolution is produced with DNA fragments ranging from 10-1500 bp.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

Maria Carmen Piñon et al.
The Journal of physiology, 587(Pt 9), 1903-1915 (2009-03-18)
In the Golli-tau-eGFP (GTE) transgenic mouse the reporter gene expression is largely confined to the layer of subplate neurons (SPn), providing an opportunity to study their intracortical and extracortical projections. In this study, we examined the thalamic afferents and layer
Víctor Carriel et al.
Journal of neural engineering, 10(2), 026022-026022 (2013-03-27)
The objective was to study the effectiveness of a commercially available collagen conduit filled with fibrin-agarose hydrogels alone or with fibrin-agarose hydrogels containing autologous adipose-derived mesenchymal stem cells (ADMSCs) in a rat sciatic nerve injury model. A 10 mm gap
Nathan A Baird et al.
PloS one, 3(10), e3376-e3376 (2008-10-15)
Single nucleotide polymorphism (SNP) discovery and genotyping are essential to genetic mapping. There remains a need for a simple, inexpensive platform that allows high-density SNP discovery and genotyping in large populations. Here we describe the sequencing of restriction-site associated DNA
Gianluca Vadalà et al.
Spine, 38(6), E319-E324 (2013-01-18)
Descriptive anatomical study on ovine and human cadaveric lumbar spinal segments. To describe the alternative transpedicular approach to deliver therapeutic agents into intervertebral disc (IVD). The present delivery approach of therapeutic agents (growth factors/cells/hydrogels) within the IVD is through injection
Michael J Lafferty et al.
The Journal of biological chemistry, 288(40), 28524-28534 (2013-08-21)
Elevated triglycerides are associated with an increased risk of cardiovascular disease, and lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of triglycerides from circulating lipoproteins. The N-terminal domain of angiopoietin-like protein 4 (ANGPTL4) inhibits LPL activity. ANGPTL4 was

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