Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

View All Documentation

A5304

Sigma-Aldrich

Agarose

Special High EEO

Synonym(s):

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

CAS Number:
9012-36-6
EC Number:
232-731-8
MDL number:
MFCD00081294
UNSPSC Code:
41105317
PubChem Substance ID:
24890968
NACRES:
NA.28

form

powder

technique(s)

electrophoresis: suitable
immunoelectrophoresis: suitable

impurities

≤11% moisture content

ash

≤1.5%

turbidity

≤4 NTU (1.5% gel)

EEO

≤0.40

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

gel strength

≥700 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.2%

storage temp.

room temp

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Suitability

Suitable for counterimmunoelectrophoresis and immunoelectrophoretic techniques with significant cathodal migration.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jean E Copper et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 208, 38-46 (2017-11-22)
In recognition of the importance of zebrafish as a model organism for studying human disease, we have created zebrafish content for a web-based reference atlas of microanatomy for comparing histology and histopathology between model systems and with humans (http://bio-atlas.psu.edu). Fixation
Marketa Kaucka et al.
eLife, 7 (2018-06-14)
Facial shape is the basis for facial recognition and categorization. Facial features reflect the underlying geometry of the skeletal structures. Here, we reveal that cartilaginous nasal capsule (corresponding to upper jaw and face) is shaped by signals generated by neural
Julia Dahlmann et al.
Biomaterials, 34(10), 2463-2471 (2013-01-22)
In most pluripotent stem cell differentiation protocols the formation of embryoid bodies (EBs) is an important step. Here we describe a rapid, straightforward soft lithography approach for the preparation of hydrophilic silicon masters from different templates and the subsequent production
Markus Landthaler et al.
RNA (New York, N.Y.), 14(12), 2580-2596 (2008-11-04)
microRNAs (miRNAs) regulate the expression of mRNAs in animals and plants through miRNA-containing ribonucleoprotein particles (RNPs). At the core of these miRNA silencing effector complexes are the Argonaute (AGO) proteins that bind miRNAs and mediate target mRNA recognition. We generated
Antoine-Emmanuel Saliba et al.
Nature methods, 11(1), 47-50 (2013-11-26)
Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service