14365C

SAFC

EX-CELL® CD CHO Fusion

Animal-component free, without L-glutamine, without hypoxanthine, without thymidine, liquid, sterile-filtered, suitable for cell culture

NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

components

L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL ® CD CHO FUSION is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. The absence of any large macromolecules allows for isolation and purification of secreted proteins from the cells.

This medium is supplied without L-glutamine to aid in media stability, to avoid L-glutamine degradation that causes ammonia build-up, and to provide an appropriate medium for the culture of CHO cells using the Glutamine Synthetase, or GS System™. This medium does not contain hypoxanthine or thymidine to allow for its use with dihydrofolate reductase (DHFR) gene amplification systems.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Qiong Wang et al.
Biotechnology and bioengineering, 116(9), 2303-2315 (2019-05-08)
With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing. Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the...
Noriko Yamano-Adachi et al.
Scientific reports, 10(1), 17612-17612 (2020-10-21)
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins....
Articles
Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines at sigmaaldrich.com
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