Animal-component free, with HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

CHO Medium
MDL number:

Quality Level


for research or for further manufacturing use






Drug/Device Master File available


cell culture | mammalian: suitable


NaHCO3: yes
HEPES: yes
L-glutamine: no
phenol red: no

shipped in


storage temp.


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Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Features and Benefits

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. Cell densities in excess of 8 × 106 cells/ml have been achieved in batch culture systems. Increases in protein productivity up to 500% per cell have been observed.

Other Notes

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.


Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC


12 - Non Combustible Liquids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Hypokalemia reduces the cardiac repolarization reserve. This prolongs the QT-interval and increases the risk of ventricular arrhythmia; a risk that is exacerbated by administration of classical class 3 anti-arrhythmic agents.Small conductance Ca2+-activated K+-channels (KCa2) are a promising new atrial selective...
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The release of soluble ligands of activating Natural Killer (NK) cell receptors may represent a regulatory mechanism of NK cell function both in physiologic and in pathologic conditions. Here, we identified the extracellular matrix protein Nidogen-1 (NID1) as a ligand...
Cell culture protocol for passaging and splitting suspension cell lines
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In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.
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A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
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The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).
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