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B4921

Sigma-Aldrich

PhastGel® Blue R

Pre-measured tablets, readily soluble.

Synonym(s):

Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
6104-59-2
Molecular Weight:
825.97
Colour Index Number:
42660
Beilstein/REAXYS Number:
5718025
EC Number:
228-060-5
MDL number:
MFCD00041762
UNSPSC Code:
12171500
PubChem Substance ID:
24891803

Quality Level

200

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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Application

Detects most proteins at 50-100 ng per band
PhastGel® Blue R has been used for the staining of protein bands after SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis). It has also been used for the staining of cell clones in the culture media plates.
For detection of protein bands following electrophoresis.

Reconstitution

One tablet makes 400 mL of a 0.1% staining solution.

Legal Information

PhastGel is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCF0442
MKCF0195
SLBQ5336V
SLBN0955V
SLBM7863V

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Pharmacokinetics and Biodistribution Study of 7A7 Anti-Mouse Epidermal Growth Factor Receptor Monoclonal Antibody and Its F(ab')(2) Fragment in an Immunocompetent Mouse Model.
Rabasa Capote A, et al.
ISRN Pharmacology, 2012, doi: 10-doi: 10 (2012)
Production of infectious HCV genotype 1b virus in cell culture using a novel Set of adaptive mutations.
Mori KI, et al.
BMC Microbiology, 16, 224-224 (2016)
Zhang Xin-Guo Chen Jian-Hua et al.
Journal of chromatographic science, 50(9), 820-825 (2012-06-22)
A human serum albumin and Thymosin α1 (HSA-Tα1) fusion protein was designed and over-expressed in Pichia pastoris. To purify the fusion protein, a new native preparative electrophoresis system that involved a modified device with a sample receiving chamber, and an
Reiner Westermeier
Proteomics, 6 Suppl 2, 61-64 (2006-10-13)
In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There
Chun Yi Liau et al.
Journal of bioscience and bioengineering, 106(1), 111-113 (2008-08-12)
A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated
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