MilliporeSigma
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1.12553

Sigma-Aldrich

Coomassie Brilliant blue R 250 (C.I. 42660)

for electrophoresis Trademark of Imperial Chemical Industries PLC

Synonym(s):
Coomassie Brilliant blue R 250 (C.I. 42660), protein stain
Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
Molecular Weight:
825.97
MDL number:
EC Index Number:
228-060-5

Quality Level

potency

>5000 mg/kg LD50, oral (Rat)

technique(s)

protein staining: suitable

pH

6.2 (25 °C, 10 g/L in H2O)

mp

174-180 °C

solubility

20 g/L

bulk density

250 kg/m3

εmax

≥300 at 554-563 nm at 0.025 g/L (pH 7.0)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30,46H,4-6,31-32H2,1-3H3,(H-,49,50,51,52,53,54);/q;+1/p-1

InChI key

HAEGGLCXLFWTBE-UHFFFAOYSA-M

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λmax. (buffer pH 7.0): 554 - 563 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.025 g/l; buffer pH 7.0; calc. on dried substance): ≥ 300
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for electrophoresis: passes test

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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