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MAB1924

Sigma-Aldrich

Anti-Laminin α5 Antibody, clone 4C7

ascites fluid, clone 4C7, Chemicon®

Synonym(s):

Anti-AA408760, Anti-AA408762, Anti-AI853660, Anti-laminin-511, Anti-mKIAA0533

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

4C7, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2a

NCBI accession no.

NM_005560.3

UniProt accession no.

O15230

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... LAMA2(3908)

Specificity

Reacts with the globular domain of the alpha5 chain of intact laminin and blocks the neurite stimulating activity of laminin. This antibody was originally thought to recognize laminin alpha 1 or A, but further characterization has confirmed its specificity for the laminin alpha 5 chain [see Tiger, C.F. (1997) J. Biol. Chem. 272: 28590].

Application

Anti-Laminin α5 Antibody, clone 4C7 is an antibody against Laminin α5 for use in ELISA, IH & IP.
ELISA (50% maximal binding to human laminin): >1:11,000.

Immunofluorescence - tissue staining pattern is most consistent with that observed for laminin alpha5 in the mouse(Miner et al., 1997)

Affinity chromatography

Immunoprecipitation

Does not work for Western blotting

Optimal working dilutions must be determined by end user.

Physical form

UnPurified mouse ascites containing no preservatives.

Analysis Note

Control
Developing striated muscle tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dysferlin associates with the developing T-tubule system in rodent and human skeletal muscle.
Klinge L, Harris J, Sewry C, Charlton R, Anderson L, Laval S, Chiu YH, Hornsey M et al.
Muscle and Nerve null
Titin mutation segregates with hereditary myopathy with early respiratory failure.
Gerald Pfeffer,Hannah R Elliott,Helen Griffin,Rita Barresi,James Miller,Julie Marsh et al.
Brain null
Y He et al.
Neurology, 57(7), 1319-1322 (2001-10-10)
The authors report a case of congenital muscular dystrophy with mild nonprogressive muscle weakness, white matter hypodensity, and absence of the laminin alpha2 chain in muscle fibers with two antibodies, but not with four others. They identified mutations in LAMA2
Jamie L Maciaszek et al.
Biophysical journal, 102(5), 1137-1143 (2012-03-13)
Collapse and sudden death in physical training are the most serious complications of sickle cell trait (SCT). There is evidence that erythrocytes in SCT patients aggregate during strenuous exercise, likely because of adhesive interactions with the extracellular matrix (ECM) and
S Matsubara et al.
Neuromuscular disorders : NMD, 9(6-7), 388-398 (1999-11-02)
Despite the recent advance in genetic study of Fukuyama-type congenital muscular dystrophy (FCMD), the mechanism of muscle degeneration in the disease remains unclear. To clarify it, muscle biopsies from six cases of FCMD were subjected to immunohistochemical and ultrastructural studies.
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