The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purification
FLAG; peptide sequence DYKDDDDK
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Browse additional application references in our FLAG® Literature portal.
Contains 50% glycerol in phosphate buffered saline.
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC