ECM506
QCM Chemotaxis Cell Migration Assay, 24-well (5 µm), colorimetric
This 3 um QCM Chemotaxis Assay 24-well plate -colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle.
Synonym(s):
Cell migration assay, Chemotaxis assay
About This Item
Recommended Products
Quality Level
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
QCM
technique(s)
activity assay: suitable
cell based assay: suitable
detection method
colorimetric
shipped in
wet ice
General description
Introduction
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (1, 2). Most cells are sized from 30 to 50 µm can migrate through 3 to 10 µm pore. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses a hollow plastic chamber, sealed at one end with a porous membrane. This chamber is suspended over a larger well which may contain medium and/or chemoattractants. Cells are placed inside the Chamber and allowed to migrate through the pores, to the other side of the membrane. Migratory cells are then stained and counted. Most migration assays utilize an 8 µm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Millipore 5 µm QCM Chemotaxis Assay 24-well- colorimetric utilizes a 5 µm pore size, which is appropriate for a subset of fibroblast cells or cancer cells such as NIH-3T3 and MDA-MAB 231 cells. Cells migrated toward chemoattractants were measured by crystal violet, a nucleic dye, and quantified by spectrophotometers. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, and chemokinesis.
The Millipore QCM 5 µm Chemotaxis Assay 24-well-colorimetric provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
• QCM 8µm 24-well Chemotaxis Cell Migration Assays (ECM508, 509)
• QCM 5µm 24-well Chemotaxis Cell Migration Assay- Fluorometric (ECM507)
• QCM 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)
• QCM 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)
• QCM 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)
• QCM 96-well ECMatrix Cell Invasion Assay (ECM555)
• QCM 96-well Collagen-based Cell Invasion Assay (ECM556)
Application
The Millipore 5 µm QCM Chemotaxis Assay 24-well-colorimetric is intended for research use only; not for diagnostic applications.
Packaging
Components
Cell Stain: (Part No. 90144) one 20 ml.
Extraction buffer: (Part No. 90145) one 20ml.
Cotton Swaps: (Part No. 10202) 50 each.
Forceps: (Part No. 10203) One each.
Storage and Stability
Legal Information
Disclaimer
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class
3 - Flammable liquids
flash_point_f
53.6 °F
flash_point_c
12 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Need A Sample COA?
This is a sample Certificate of Analysis (COA) and may not represent a recently manufactured lot of this specific product.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service