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ABN1009

Sigma-Aldrich

Anti-TRPA1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Transient receptor potential cation channel subfamily A member 1, TRPA1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_015628.2

UniProt accession no.

O75762

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TRPA1(8989)

General description

Transient receptor potential A1 (also known as Transient receptor potential ankyrin 1) is an excitatory ion channel on primary sensory nerves of the pain pathway. It is encoded by the TRPA1/ANKTM1 gene in human (Trpa1/Anktm1 in rat & mouse) and plays a critical role in pain associated with tissue injury, inflammation, and nerve lesions. TRPA1 is reported to colocalize with PAR2 and coexpress with TRPV1 (Transient receptor potential vanilloid 1) in rat dorsal root ganglion (DRG) neurons. Agonist-induced TRPA1 current is shown to be enhanced upon PAR2 cleavage/activation by proinflammatory trypsin & tryptase (PMID 17571167). TRPA1 activation, in turn, is reported to result in enhanced TRPV1 phosphorylation and activity in a calcium- and PKA-dependent manner (PMID 24275229).

Immunogen

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TRPA1.

Application

Anti-TRPA1 Antibody is an antibody against TRPA1 for use inWestern Blotting and Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected TRPA1 in rat cerebellum, rat hindbrain, and rat pancreas tissue.

Quality

Evaluated by Western Blotting in rat brain membrane tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected TRPA1 in 10 µg of rat brain membrane tissue lysate.

Target description

~130 kDa observed. Uncharacterized band(s) may appear in some lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Min Xia et al.
Frontiers in molecular neuroscience, 12, 98-98 (2019-05-07)
Intracerebral hemorrhage (ICH) is a devastating disease that is characterized by high morbidity and high mortality. ICH has an annual incidence of 10-30/100,000 people and accounts for approximately 10%-30% of all types of stroke. ICH mostly occurs at the basal

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