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537060

Sigma-Aldrich

Propidium Iodide Solution - CAS 25535-16-4 - Calbiochem

A convenient form of propidium iodide useful for flow cytometry studies.

Synonym(s):
Propidium Iodide Solution - CAS 25535-16-4 - Calbiochem
Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
MDL number:

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

input

sample type intact cells

fluorescence

λex 536 nm
λem 617 nm

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

Related Categories

General description

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

Packaging

5 ml in Plastic ampoule

Biochem/physiol Actions

Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Warning

Toxicity: Standard Handling (A)

Physical form

50 µg/ml in PBS, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C).

Other Notes

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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