All Photos(1)

81845

Sigma-Aldrich

Propidium iodide

≥94% (HPLC)

Synonym(s):
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
Beilstein:
3843838
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.77

Quality Level

assay

≥94% (HPLC)

mp

220-225 °C (dec.) (lit.)

fluorescence

λex 482 nm; λem 608 nm (pH 7.2)
λex 540 nm; λem 608 nm (bound to DNA)

storage temp.

2-8°C

SMILES string

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

General description

Propidium iodide is a fluorescent intercalating agent used for staining. It functions as an apoptosis marker and has linear DNA-binding capacity. Propidium iodide is an effective indicator of tissue injury.
PI is used for selective labelling of dead cells.

Application

in scrape-loading dye transfer assay
Propidium iodide has been used:
  • to stain DNA in cell cycle analysis
  • to stain cells and cell nuclei
  • for the preparation of propidium iodide (PI)stock solution
  • to stain cells for flow cytometry

Packaging

25, 100 mg in glass bottle
Bottomless glass bottle. Contents are inside inserted fused cone.

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Muta. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

More documents

Quotes and Ordering

Oxygen tension-controlled matrices with osteogenic and vasculogenic cells for vascularized bone regeneration in vivo
Amini AR, et al.
Tissue Engineering: Part A, 22(7-8), 610-620 (2016)
Silibinin Protects Ultraviolet B-Irradiated Skin by Balancing Apoptosis and Autophagy in Epidermis and Dermis
Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging, 401-418 (2017)
Intravital microscopic methods to evaluate anti-inflammatory effects and signaling mechanisms evoked by hydrogen sulfide
Methods in Enzymology, 555, 93-125 (2015)
High-throughput, non-perturbing quantification of lipid droplets with Digital Holographic Microscopy
Campos V, et al.
Journal of Lipid Research, jlr-D085217 (2018)
Flow cytometry (2008)

Related Content

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.

Discover Bioactive Small Molecules for Apoptosis

Apoptosis, or programmed cell death (PCD), is a selective process for the removal of unnecessary, infected or transformed cells in various biological systems. As it plays a role in the homeostasis of multicellular organisms, apoptosis is tightly regulated through two principal pathways by a number of regulatory and effector molecules.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service