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17-295

Sigma-Aldrich

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Synonym(s):
Agarose ChIP Kit
eCl@ss:
32161000
NACRES:
NA.41

Quality Level

application(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

Related Categories

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Components

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Quality

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Certificate of Analysis

Certificate of Origin

M Vignali et al.
The EMBO journal, 19(11), 2629-2640 (2000-06-03)
We analyzed the targeting of histone acetyltransferase (HAT) complexes by DNA-binding activators during transcriptional activation and the resulting distribution of acetylated histones. An in vitro competition assay was developed to acetylate and transcribe a nucleosomal array template in the presence...
Seed-based systematic discovery of specific transcription factor target genes.
Mrowka, R; Bluthgen, N; Fahling, M
FEBS Journal null
Bovine Foamy Virus Transactivator (BTas) Interacts with Cellular RelB to Enhance Viral Transcription.
Wang J, Tan J, Guo H, Zhang Q, Jia R, Xu X, Geng Y, Qiao W
Journal of virology null
Regulation of human papillomavirus type 31 gene expression during the differentiation-dependent life cycle through histone modifications and transcription factor binding.
Tonia R Wooldridge, Laimonis A Laimins
Virology null
Chromatin immunoprecipitation for identifying transcription factor targets in keratinocytes.
Kori Ortt,Satrajit Sinha
Methods in Molecular Biology null

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