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16-661

Sigma-Aldrich

Magna ChIP® Protein A Magnetic Beads

Recombinant Protein A covalently bound to magnetic beads for use in chromatin immunoprecipitations (ChIP assays). These protein A beads provide users a more rapid, reproducible & efficient reagent for collecting immunocomplexes vs. agarose beads.

Synonym(s):
ChIP magnetic beads, ChIP magnetic A beads
eCl@ss:
32160405

Quality Level

packaging

pkg of 1 mL

storage condition

do not freeze

particle size

~3 μm

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Recombinant protein A covalently bound to magnetic beads. These beads provide users a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitations (ChIP) assays. Compared with conventional protein A agarose beads, protein A magnetic beads significantly reduce the handling time and mechanical stress on target immunocomplexes.

Application

Research Category
Epigenetics & Nuclear Function
Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.
Used to detect/quantify: Protein A

Quality

Routinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP® A Kit (Cat. #17-610).

Physical form

Liquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of shipment. Do Not Freeze.

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Gerda Lagger et al.
Molecular and cellular biology, 23(8), 2669-2679 (2003-04-01)
The cyclin-dependent kinase inhibitor p21/WAF1/CIP1 is an important regulator of cell cycle progression, senescence, and differentiation. Genotoxic stress leads to activation of the tumor suppressor p53 and subsequently to induction of p21 expression. Here we show that the tumor suppressor...
Distinct roles for Sp1 and E2F sites in the growth/cell cycle regulation of the DHFR promoter.
Jensen, D E, et al.
Journal of Cellular Biochemistry, 67, 24-31 (1997)

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