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一般說明
Formerly E.C.1.1.3.22
黄嘌呤氧化酶(XOD)是一种金属黄素蛋白。含黄素腺嘌呤二核苷酸(FAD)、钼和铁,三者比例为 2:2:8。此同二聚体的分子量为290kDa。黄嘌呤氧化酶是钼蛋白家族成员,由两个分离的底物结合位点组成。
應用
牛乳中提取的黄嘌呤氧化酶用于:
- 制备黄嘌呤氧化酶(XO)溶液 ,用于5-(二乙氧基磷酰)-5-甲基-1-吡咯啉N-氧化物(DEPMPO)自旋捕获分析
- 体外 XO检测,筛选越南药用植物XO抑制活性
- 确定XO活性的标准品
- 检测二十二碳六烯酸(DHA)协同效应的标准品
生化/生理作用
木糖氧化酶是一种含钼酶,存在于细胞质中,能够被黄酮类化合物有效抑制。它在一些药物和嘌呤嘧啶代谢中有重要作用。它被认为是活性氧簇的生物来源。
木糖氧化酶通过产生超氧阴离子然后氧化木糖,参与细胞色素c还原。这些自由基能够还原色素c。
黄嘌呤氧化酶(XO)催化将次黄嘌呤羟基化为黄嘌呤,将黄嘌呤羟基化为尿酸。
單位定義
一单位每分钟能够将1.0 μmole木糖转化成尿酸,PH7.5,25℃。次黄嘌呤作为底物,活性大约是50%。
外觀
在含有1mM 水杨酸钠的2.3 M (NH4)2SO4中复溶
分析報告
双缩脲法测定的蛋白
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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