推薦產品
形狀
lyophilized powder
品質等級
比活性
≥1500 units/mg protein
分子量
32.5 kDa
成份
Protein, ≥70% biuret
UniProt登錄號
儲存溫度
−20°C
基因資訊
cow ... SOD1(281495)
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一般說明
研究领域:细胞信号传导 超氧化物歧化酶(SOD)是一种低分子量蛋白,存在于微生物、植物和动物的所有需氧细胞中。它以三个不同家族的形式存在,如锰SOD、铜–锌SOD和胞外SOD。
應用
来自牛肝的超氧化物歧化酶已被用于一项研究中,用于确定高胆固醇血症可以增加内皮细胞超氧化物阴离子的生成。 来自牛肝的超氧化物歧化酶也已被用于研究牛红细胞超氧化物歧化酶的重氮偶联、亚基相互作用和电泳变体。它也被用作O2k 荧光法定量测定活性氧(ROS)的分析缓冲液成分。
生化/生理作用
超氧化物歧化酶催化超氧化物自由基的歧化反应生成过氧化氢和分子氧。它在细胞防御氧自由基的毒性作用方面发挥着重要作用。它 与一氧化氮(NO)竞争超氧化物阴离子(可与NO反应生成过亚硝酸盐),因此SOD能促进NO的活性。在培养的大鼠卵泡、神经细胞系和转基因小鼠中,SOD也表现出抑制细胞凋亡的作用。
單位定義
在 25℃、pH 7.8 的黄嘌呤氧化酶偶联系统中,1 个单位将抑制细胞色素 c 减少 50%(反应体积 3.0 mL)。黄嘌呤氧化酶浓度应每分钟 产生 0.025±0.005 初始 ΔA 550 。
外觀
含磷酸钾缓冲盐的冻干粉
分析報告
关于试验方法请参阅 McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969)。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
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