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S7571

Sigma-Aldrich

超氧化物歧化酶 来源于牛红细胞

greener alternative

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

同義詞:

红细胞铜蛋白, 细胞铜蛋白, 血铜蛋白, 超氧化物歧化酶1, 牛, 铜/锌-SOD, SOD, 超氧化物:超氧化物氧化还原酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bovine

品質等級

形狀

lyophilized powder

比活性

≥3,000 units/mg protein

分子量

32.5 kDa

成份

Protein, ≥95% biuret

儲存條件

(Store under nitrogen.
Tightly closed. Dry.)

環保替代產品特色

Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

immunoblotting: suitable
inhibition assay: suitable

顏色

blue-green

pI 

4.95

溶解度

water: 20 mg/mL
aqueous buffer, pH 7.5: soluble

UniProt登錄號

應用

diagnostic assay manufacturing

環保替代類別

儲存溫度

−20°C

基因資訊

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相關類別

一般說明

研究领域:细胞信号传导

超氧化物歧化酶(SOD)是一种具有氧化还原活性的金属酶,在有氧和厌氧生物体内均有表达。牛超氧化物歧化酶或CuZn SOD是一种同源二聚体,每个亚基含有一个锌离子和一个铜离子。

應用

红细胞超氧化物歧化酶已用于:

  • 在一项研究评估辐射诱导失活的超氧化物歧化酶在一氧化二氮饱和溶液中的动力学模型
  • 研究超氧阴离子参与肠色氨酸2,3-双加氧酶反应的可能性
  • 调查其对人红细胞溶血率及红细胞血红蛋白-一氧化氮复合物(HbNO)稳定性的影响
  • 联合过氧化氢酶体外研究其对细胞分化的影响
  • 通过化学发光和细胞色素C还原法量化超氧化物水平,研究其对小鼠肺动脉反应性的影响

生化/生理作用

牛红细胞来源的超氧化物歧化酶可催化超氧化物自由基歧化为过氧化氢和分子氧。作为一种抗氧化剂,它在保护细胞免受氧自由基毒性作用方面起着至关重要的作用。与一氧化氮(NO)竞争超氧化物阴离子(可与NO反应生成过亚硝酸盐),因此SOD能促进NO的活性。在培养的大鼠卵泡、神经细胞系和转基因小鼠中,SOD也表现出抑制细胞凋亡的作用。

單位定義

在 3.0ml 反应体系、25℃、pH 7.8 条件下,在与黄嘌呤氧化酶的偶联系统中,一个单位将抑制 50% 的细胞色素 c 的还原。黄嘌呤氧化酶浓度应每分钟产生 0.025±0.005 的初始 ΔA550

外觀

冻干粉末,基本上不含盐

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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存取文件庫

Possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction.
F Hirata et al.
The Journal of biological chemistry, 246(24), 7825-7826 (1971-12-25)
Sebastian Overmans et al.
Aquatic toxicology (Amsterdam, Netherlands), 198, 165-174 (2018-03-20)
Exposure to polycyclic aromatic carbons (PAHs) poses a growing risk to coral reefs due to increasing shipping and petroleum extraction in tropical waters. Damaging effects of specific PAHs can be further enhanced by the presence of ultraviolet radiation, known as
Colleen M Hansel et al.
mBio, 10(2) (2019-03-14)
There is a growing appreciation within animal and plant physiology that the reactive oxygen species (ROS) superoxide is not only detrimental but also essential for life. Yet, despite widespread production of extracellular superoxide by healthy bacteria and phytoplankton, this molecule
C A Chuaqui et al.
Biochimica et biophysica acta, 702(1), 112-116 (1982-03-18)
It has been reported that the inactivation yield of superoxide dismutase from bovine erythrocytes irradiated in N2O-saturated solutions increases exponentially with the initial enzyme concentration and that dose-response curves are non-exponential. The present work analyses these data using a kinetic
Tomohiro Furukawa et al.
Toxins, 11(2) (2019-02-15)
Aflatoxin contamination of crops is a worldwide problem, and elucidation of the regulatory mechanism of aflatoxin production, for example relative to the oxidative⁻antioxidative system, is needed. Studies have shown that oxidative stress induced by reactive oxygen species promotes aflatoxin production.

文章

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

條款

Enzymatic Assay of Superoxide Dismutase

Chromatograms

application for HPLC

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