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一般說明
卷心菜磷脂酶D(PLD)包含C2结构域和带HxKx4Dx6GSxN(HKD)基序的PLD超家族特异性保守区域(I–IV)。结构上是一种连接钙离子的桶状单体。
應用
在荧光标记脂质制备过程中，该酶已可用于制备氟硼吡咯-磷脂酰胆碱。
磷脂酶D(PLD)水解磷脂与鞘磷脂的磷酸键，得到相应的磷脂酸。它已可用于研究代谢标记和 体内 直接成像胆碱磷脂，通过测量propargyl-cho的掺入。 此外，PLD已可用于纯化和动力学研究。
生化／生理作用
卷心菜磷脂酶D(PLD)的氨基酸序列与拟南芥PLDα和蓖麻子PLD的一致性分别为83%和80%。 磷脂酶D水解磷脂和鞘磷脂的磷酸键，从而得到相应的磷脂酸。
磷脂酶D（来源于卷心菜）广泛用于生物催化的转磷脂酰反应。
水解磷脂和鞘磷脂的磷酸键，获得相应的磷脂酸。
單位定義
在pH为5.6，温度为30°C的条件下，一个单位酶每小时可从 L-α-磷脂酰胆碱（蛋黄）中释放1.0 μmole的胆碱。
分析報告
蛋白质为双缩脲法测定。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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