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Key Documents

P6534

Sigma-Aldrich

磷脂酶 A2 来源于猪胰腺

ammonium sulfate suspension, ≥600 units/mg protein

同義詞:

PLA, 卵磷脂酶 A, 磷脂酰胆碱 2-酰基水解酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

形狀

ammonium sulfate suspension

品質等級

比活性

≥600 units/mg protein

UniProt登錄號

儲存溫度

2-8°C

基因資訊

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一般說明

磷脂酶 A2 是一种富含二硫键的小分子蛋白,有 124 个残基。 是一种钙依赖性酶。

應用

磷脂酶 A2 已用于磷脂酶试验,并确定大鼠肾近曲小管段 (PTS) 在氧合和缺氧-复氧过程中的活性。

生化/生理作用

与单体底物相比,它对聚集的底物具有较高的催化活性。
水解 β-两性甘油磷脂的酯键。首选底物为磷脂酰胆碱、磷脂酰乙醇胺及其缩醛磷脂类似物。磷脂酰肌醇和磷脂酰丝氨酸也被水解。其会攻击完整细胞膜上的磷脂。

單位定義

在 pH 8.0、37°C 条件下,1 个单位每分钟将 1.0 μmol 大豆 L-α-磷脂酰胆碱水解为 L-α-溶血磷脂酰胆碱和 1 个脂肪酸。

外觀

在 3.2 M (NH 4 ) 2 SO 4 溶液 (pH 5.5) 中的混悬液

分析報告

双缩脲法测定蛋白质。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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B van den Berg et al.
The EMBO journal, 14(17), 4123-4131 (1995-09-01)
The lipolytic enzyme phospholipase A2 (PLA2) is involved in the degradation of high-molecular weight phospholipid aggregates in vivo. The enzyme has very high catalytic activities on aggregated substrates compared with monomeric substrates, a phenomenon called interfacial activation. Crystal structures of
Knut Kölbel et al.
Biophysical chemistry, 206, 12-21 (2015-06-29)
Porcine pancreatic phospholipase A2, a small and disulfide rich protein, is extremely resistant against chemically or thermally induced unfolding. Despite this marked resistance, the protein displays broad unfolding transitions resulting in comparatively low apparent thermodynamic stability. Broad unfolding transitions may
R A Zager et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8297-8301 (1993-09-01)
During hypoxic or ischemic renal tubular injury, phospholipase A2 (PLA2) induces membrane deacylation, causing fatty acid accumulation and phospholipid breakdown. Because these changes can compromise cellular integrity, PLA2 activity has been widely proposed as a critical mediator of hypoxic renal
Elena Venuti et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 16(6), 763-770 (2017-07-26)
Bile salt stimulated lipase (BSSL; Enzyme Commission (EC) number 3.1.1.13) has been a candidate triglyceridase for improving enzyme therapy for pancreatic insufficiency; however, its efficacy is near absent. We hypothesise that similarly to pancreatic lipase, BSSL is inhibited by phospholipids
B van den Berg et al.
Journal of biomolecular NMR, 5(2), 110-121 (1995-02-01)
The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present in a 40 kDa ternary complex with micelles and a competitive inhibitor, has been determined using multidimensional heteronuclear NMR spectroscopy. The structure of the protein (124 residues) is based on 1854

文章

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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