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L0382

Sigma-Aldrich

脂肪酶 来源于猪胰腺

Type VI-S, ≥20,000 units/mg protein, lyophilized powder

同義詞:

PPL, 三酰基甘油脂肪酶, 三酰基甘油酰基水解酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type VI-S

形狀

lyophilized powder

比活性

≥20,000 units/mg protein

成份

Protein, 40-70%

運輸包裝

wet ice

儲存溫度

−20°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI 密鑰

QWZUIMCIEOCSJF-CHHCPSLASA-N

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一般說明

来自猪胰腺的脂肪酶(PPL)或猪胰腺脂肪酶是小型球状蛋白,N-端含有αβ型折叠区域。另含有催化三联体Ser、Asp和His残基和C端β-三明治结构域。猪胰腺脂肪酶属于羧酸酯酶家族的亚家族。

應用

猪胰腺脂肪酶已用于:
  • 植物提取物的脂肪酶抑制分析
  • 糊化淀粉/纤维混合物的体外消化
  • 加入模拟肠液以模拟消化道条件,用于发酵燕麦中的发酵乳杆菌(L. fermentum)PC1的继续存活

脂肪酶在工业上用于拆分手性化合物和生物柴油的酯交换生产。

生化/生理作用

猪胰腺脂肪酶(PPL)可催化脂肪(脂质)水解。它在大多数生物体的膳食脂质(如甘油三酯、脂肪、油脂)消化、转运和加工中起重要作用。猪胰腺脂肪酶具有高性价比,可用于生物转化反应。它具有高选择性,并具有催化活性。猪胰腺脂肪酶还可掺入去污剂,用于去除衣物上的油渍。
脂肪酶可催化水溶液中酯的水解和非水溶液中酯的合成。它们通过水解聚对苯二甲酸乙二酯中的酯键,产生羟基和羧基。

脂肪酶可催化三酰甘油的水解,生成甘油和游离脂肪酸。

單位定義

一个酶活性单位是指在pH 7.7、温度37 °C条件下,以橄榄油为底物,在1小时内水解甘油三酸酯而释放1.0微当量的脂肪酸所需的酶量。

分析報告

通过缩二脲法测定的蛋白质。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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