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一般說明
米根霉脂肪酶(ROL)由一个氧阴离子洞、四个 N-糖基化位点和一个活性位点组成。它具有 N-端前序列和前序列。
Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.
應用
使用米根霉脂肪酶:
- 测试其对 1,2-甘油二油酸酯合成和甘油三油酸酯乙醇分解的影响
- 固定氧化石墨烯载体,以便于生物催化研究
- 从莱茵衣藻和酿酒酵母中消化甘油三酯(TAG)
脂肪酶在工业上用于拆分手性化合物和生物柴油的酯交换生产。
生化/生理作用
米根霉脂肪酶(ROL)作为一种催化剂,用于通过水解逆转酶促合成聚甘油蓖麻醇酯。基于其 1,3-区域特殊性功能,ROL 在结构化脂质的工业生产中十分有用。
Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.
甘油三酸酯、甘油二酸酯和甘油单酸酯被水解(按降序排列)。
脂肪酶可催化三酰基甘油水解成甘油和游离的脂肪酸。
脂肪酶可催化三酰基甘油水解成甘油和游离的脂肪酸。
單位定義
1 U 相当于 pH 8.0,40°C 下每分钟释放 1 μmol 丁酸的酶量(三丁酸甘油酯,货号 91010,作为底物)如上所述,5000 U 相当于 ~1 U,使用三油酰甘油酯,货号 62314,作为底物,在 pH 8.0,40°C 下
其他說明
说明:以三乙酰甘油酯作为底物时,pH 为 7.4。孵化期:60 分钟。
催化油和脂肪的酯交换反应;用于去除电免疫测定载脂蛋白 B 中的干扰甘油三酯;通过对映选择性酶水解拆分外消旋环氧酯
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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