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Key Documents

P4912

Sigma-Aldrich

磷脂酶 D 来源于链霉菌

Type VII, lyophilized powder, ≥150 units/mg solid

同義詞:

Phosphatidylcholine phosphatidohydrolase, 卵磷脂酶 D

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Streptomyces sp.

品質等級

種類

Type VII

形狀

lyophilized powder

比活性

≥150 units/mg solid

儲存溫度

−20°C

應用

体外 条件下,Sigma的该酶被用于鞘氨酸激酶1 (SK1)向膜组分的转移。它还被用于从HL60透化性细胞中的磷脂酰胆碱(PC)产生磷脂酸(PA)。
脂酶D(PLD)已被用作水解磷脂与鞘磷脂的磷酸键,得到相应的磷脂酸。 它已可用于研究代谢标记和 体内 直接成像胆碱磷脂,通过测量propargyl-cho的掺入。此外,PLD已可用于纯化和动力学研究。

生化/生理作用

磷脂酶D是甘油磷脂特异性的。 它对鞘磷脂和溶血磷脂的活性明显降低。 磷脂酶D水解磷脂和鞘磷脂的磷酸键,从而得到相应的磷脂酸。
水解磷脂和鞘磷脂的磷酸键,获得相应的磷脂酸。

包裝

无底玻璃瓶。内容物在插入的熔锥内。

單位定義

在pH为 8.0,温度是37 °C的条件下,一个单位酶每小时可从1,2-二油基-{1}sn{2}-甘油-3-磷酸胆碱释放1.0 μmole的胆碱。

分析報告

蛋白质由双缩脲法测定

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Mohamed H Yaghmour et al.
Journal of lipid research, 62, 100022-100022 (2021-01-17)
Phospholipids with a choline head group are an abundant component of cellular membranes and are involved in many important biological functions. For studies on the cell biology and metabolism of these lipids, traceable analogues where propargylcholine replaces the choline head
Kathryn Garner et al.
The Journal of biological chemistry, 287(38), 32263-32276 (2012-07-24)
Phosphatidylinositol transfer proteins (PITPs) are versatile proteins required for signal transduction and membrane traffic. The best characterized mammalian PITPs are the Class I PITPs, PITPα (PITPNA) and PITPβ (PITPNB), which are single domain proteins with a hydrophobic cavity that binds
Christine Delon et al.
The Journal of biological chemistry, 279(43), 44763-44774 (2004-08-18)
Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Because both substrate and product of the enzyme are potentially important signaling molecules, the regulation of SK1 is of considerable interest. We report that SK1, which is ordinarily a
Franka Voigt et al.
RNA (New York, N.Y.), 18(12), 2128-2134 (2012-10-23)
Piwi-interacting RNAs (piRNAs) are a gonad-specific class of small RNAs that associate with the Piwi clade of Argonaute proteins and play a key role in transposon silencing in animals. Since biogenesis of piRNAs is independent of the double-stranded RNA-processing enzyme
Milya Davlieva et al.
Antimicrobial agents and chemotherapy, 57(1), 289-296 (2012-11-02)
Daptomycin (DAP) resistance in enterococci has been linked to mutations in genes that alter the cell envelope stress response (CESR) (liaFSR) and changes in enzymes that directly affect phospholipid homeostasis, and these changes may alter membrane composition, such as that

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