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重要文件

L9764

Sigma-Aldrich

脂多糖(粗糙菌株) 来源于肠沙门氏菌 明尼苏达血清型 Re 595(Re 突变体)

同義詞:

LPS

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About This Item

MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25

生物源

Salmonella enterica (Serotype minnesota Re 595 mutant)

品質等級

形狀

lyophilized powder

雜質

<3% Protein (Lowry)

顏色

white to yellow cast

溶解度

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

本品由沙门氏菌菌种明尼苏达粒径Re 595(Re突变体)采用酚-氯仿-石油醚提取程序制备而成。来源菌株为 ATCC 49284。菌株 Re 595 的 LPS 中的一种独特结构显示可诱导人血小板分泌和聚集。

應用


  • 电荷和聚集模式决定了塑化素与模拟革兰氏阴性菌外膜外叶的LPS单层的相互作用:该研究探讨了电荷和聚集模式如何影响塑化素与来自肠道沙门氏菌血清型Minnesota Re 595等粗糙菌株的脂多糖(LPS)相互作用。这些发现有助于理解微生物耐药机制中涉及的分子相互作用(Michel et al., 2015)。

生化/生理作用

脂多糖(LPS)位于膜的外层,并且在非包封的菌株中,其暴露于细胞表面。它们有助于外膜的完整性,并可保护细胞免受胆汁盐和亲脂性抗生素的作用。

準備報告

通过苯酚-氯仿-石油醚萃取进行制备。

本品可溶于水(5 mg/ml)或细胞培养基(1 mg/ml),产生混浊的淡黄色溶液。溶于生理盐水并涡旋和加热至70-80 oC后,可形成浓度更高但仍然混浊的溶液(20 mg/ml)。脂多糖分子可在各种溶剂中形成胶束。溶于水和磷酸盐缓冲盐水时,可观察到浑浊的溶液。使用有机溶剂不能形成更清晰的溶液。使用甲醇可产生具有漂浮物的混浊悬浮液,而溶于水会产生均匀的混浊溶液。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

防範說明

危險分類

Acute Tox. 4 Oral

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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