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重要文件

L6511

Sigma-Aldrich

脂多糖 来源于肠沙门氏菌 肠道血清型

purified by phenol extraction

同義詞:

LPS(脂多糖)

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About This Item

MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25

生物源

Salmonella enterica (Serotype typhimurium)

品質等級

形狀

lyophilized powder

純化經由

phenol extraction

雜質

<3% Protein (Lowry)

顏色

white to faint yellow

溶解度

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

本品为从鼠伤寒沙门菌中提取的苯酚。来源菌株为 ATCC 7823。该 LPS 已被用于诱导大鼠和豚鼠的 NOS。

應用

脂多糖(LPS)是革兰氏阴性细菌细胞壁的特征组分。LPS及其脂质A部分通过Toll样受体4(TLR4)刺激先天免疫系统的细胞,Toll样受体 4是Toll样受体蛋白家族的成员,其识别常见的病原体相关分子模式(PAMP)。

生化/生理作用

脂多糖 (LPS) 定位于膜的外层,在无荚膜的菌株中暴露于细胞表面。它们有助于外膜的完整性,并保护细胞免受胆盐和亲脂性抗生素的操作。

準備報告

本品可溶于水 (5 mg/mL) 或细胞培养基 (1 mg/mL),得到淡黄色浑浊溶液。涡旋并加热至 70-80 oC 后,在盐水中获得了更浓缩但仍浑浊的溶液 (20 mg/mL)。脂多糖是在每种溶剂中形成胶束的分子。在水和磷酸盐缓冲盐水中观察到浑浊溶液。有机溶剂不能提供更清晰的溶液。甲醇产生浑浊的带有悬浮物的混悬液,而水产生均匀的浑浊溶液。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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Sanjoy Kumar Bhunia et al.
Carbohydrate research, 345(17), 2542-2549 (2010-10-12)
A water-soluble polysaccharide, isolated from the hot water extract of the fruiting bodies of an edible mushroom, Lentinus squarrosulus (Mont.) Singer was found to contain d-galactose, l-fucose, and d-glucose in a molar ratio of nearly 1:1:5. Structural investigation of the
C Marcq et al.
Poultry science, 90(1), 59-67 (2010-12-24)
In this study, Salmonella enterica serovar Typhimurium challenge models were tested to identify the best conditions under which to perform the experimental infection of 3-wk-old broilers. Such a model would be useful to study the efficiency of therapeutic treatments that
Alejandro Carrillo-Jimenez et al.
Frontiers in cellular neuroscience, 12, 313-313 (2018-10-10)
Microglia, the resident immune cells of the brain, have multiple functions in physiological and pathological conditions, including Alzheimer's disease (AD). The use of primary microglial cell cultures has proved to be a valuable tool to study microglial biology under various
Li Liang et al.
Scientific reports, 3, 1043-1043 (2013-01-11)
Current serological diagnostic assays for typhoid fever are based on detecting antibodies against Salmonella LPS or flagellum, resulting in a high false-positive rate. Here we used a protein microarray containing 2,724 Salmonella enterica serovar Typhi antigens (>63% of proteome) and
Andrej Kovac et al.
Journal of neuroinflammation, 8, 139-139 (2011-10-15)
Brain microvascular pericytes are important constituents of the neurovascular unit. These cells are physically the closest cells to the microvascular endothelial cells in brain capillaries. They significantly contribute to the induction and maintenance of the barrier functions of the blood-brain

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