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重要文件

L7658

Sigma-Aldrich

LB 肉汤 (Lennox)

EZMix powder microbial growth medium

同義詞:

Lennox broth

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

等級

for molecular biology

品質等級

描述

quick-dissolve, dust-free formulation

無菌

non-sterile

形狀

powder

技術

microbiological culture: suitable

pH值

6.8-7.2(2.5% solution)

應用

agriculture
food and beverages
microbiology

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

一般說明

Lennox LB是一种被高度引用的微生物生长培养基,适用于培养大肠杆菌。这种营养丰富的微生物肉汤采用低盐配方,含有多肽、氨基酸、水溶性维生素和碳水化合物。

應用

适用于大肠杆菌菌株的非选择性培养,用于克隆、DNA质粒生产和重组蛋白生产。当加入适当的抗生素时,也适用于选择性培养,包括需要低盐条件的抗生素,如博莱霉素®

特點和優勢

Lennox LB EZMix粉末提供:
  • 无尘颗粒,便于安全操作和更快混合
  • 便捷式小包装,无需称重步骤
  • 使用大包装可轻松放大规模
  • 标准配方

成分

*请注意,某些产品规格以液体形式提供。本品为粉末形式,并且这些规格反映了最终的重悬体积。

10g/L 胰蛋白胨
5 g/L 酵母提取物
5 g/L NaCl
0.6 g/L 惰性粘合剂(仅限EZMix配方)

準備報告

1.将20.6 g悬浮于1L蒸馏水中。
2.在121℃下高压蒸汽灭菌15分钟。
制备LB肉汤:加入1g 葡萄糖,再按上述制备说明操作。
制备Enquist和Sternberg培养基:高压蒸汽灭菌后,无菌操作下加入10 ml无菌1M硫酸镁。

重構

搅拌,使20.6g粉末悬浮于1L水中。在121°C下高温灭菌15分钟以灭菌。先冷却再添加其他成分,如抗生素(如果需要)。

法律資訊

Zeocin is a registered trademark of Cayla Sarl

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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Jennifer White et al.
Protein science : a publication of the Protein Society, 13(9), 2406-2415 (2004-08-24)
Decay-accelerating factor (DAF, CD55) is a glycophosphatidyl inositol-anchored glycoprotein that regulates the activity of C3 and C5 convertases. In addition to understanding the mechanism of complement inhibition by DAF through structural studies, there is also an interest in the possible
Katherine A Kantardjieff et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 5), 895-902 (2004-04-23)
The Mycobacterium tuberculosis rmlC gene encodes dTDP-4-keto-6-deoxyglucose epimerase, the third enzyme in the M. tuberculosis dTDP-L-rhamnose pathway which is essential for mycobacterial cell-wall synthesis. Because it is structurally unique, highly substrate-specific and does not require a cofactor, RmlC is considered
Michael C Konopka et al.
Journal of bacteriology, 188(17), 6115-6123 (2006-08-23)
The first in vivo measurements of a protein diffusion coefficient versus cytoplasmic biopolymer volume fraction are presented. Fluorescence recovery after photobleaching yields the effective diffusion coefficient on a 1-mum-length scale of green fluorescent protein within the cytoplasm of Escherichia coli
Alexis Kaushansky et al.
Nature protocols, 5(4), 773-790 (2010-04-03)
Protein microarrays provide an efficient way to identify and quantify protein-protein interactions in high throughput. One drawback of this technique is that proteins show a broad range of physicochemical properties and are often difficult to produce recombinantly. To circumvent these
Feng Xu et al.
PloS one, 6(4), e19344-e19344 (2011-05-10)
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques

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